I just learned to use python (and Biopython) so this question may bespeak my inexperience.
In order to carry out MSA of sequences in a file (FileA.fasta), I use the following code:
from Bio.Align.Applications import MuscleCommandline
inp = 'FileA.fasta'
outp = 'FileB.fasta'
cline = MuscleCommandline(input=inp, out=outp)
cline()
I get the following error:
ApplicationError
...
Non-zero return code 127 from 'muscle -in FileA.fasta -out FileB.fasta', message '/bin/sh: muscle: command not found'
I know that this has something to do with the executable not being in my working PATH. The Biopython tutorial suggests that I update the PATH to include the location of Muscle Tools and it gives an example of this for Windows, but I don't know how to do this for MAC.
Please help.
Thank you.
First make sure you know where you installed muscle
. If, for example, you installed muscle in:
/usr/bin/muscle3.8.31_i86darwin64
then you edit /etc/paths
with:
$ sudo vi /etc/paths
Each entry is separated by line breaks:
/usr/local/bin
/bin
/usr/sbin
/sbin
Add the appropriate path (in this example /usr/bin
) to the list. Save with wq!
Now, make sure the muscle
is on your path. Try to run
muscle -in FileA.fasta -out FileB.fasta
If that works, the BioPython code should work as well.