determining the properties of pores
I have several series of segmented images and I need to calculate the properties (area, perimeter, radius of the largest circle fitting in area) of the black areas (pores). I could not find the value with the software imagej as it seems that it has some problems in distinguishing the holes (569 area was found) and I tried to find the values by regionprops in matlab. however, it seems that it has the same problem (570 area was found). So, can anybody please help me find the solution to this question? here is the areas found by imagej.
The black regions bleed into one another and ImageJ cannot distinguish them. If you use Process > Binary > Watershed, this will segregate the regions into (most likely) separate objects. Be aware that you may need to invert the image before doing this. Then you can use Analyze > Analyze Particles... to find the objects. The measurements that ImageJ produces can be controlled using Set Measurements and in the dialog for Analyze Particles. It will do area and perimeter for each object, but not (AFAIK) the radius of the largest circle.
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