I'm dealing with several outputs I obtain from QIIME, texts which I want to manipulate for obtaining boxplots. Every input is formatted in the same way, so the manipulation is always the same, but it changes the source name. For each input, I want to extract the last 5 rows, have a mean for each column/sample, associate the values to sample experimental labels (Group) taken from the mapfile and put them in the order I use for making a boxplot of all the 6 data obtained.
In bash, I do something like "for i in GG97 GG100 SILVA97 SILVA100 NCBI RDP; do cp ${i}/alpha/collated_alpha/chao1.txt alpha_tot/${i}_chao1.txt; done
" to do a command various times changing the names in the code in an automatic way through ${i}
.
I'm struggling to find a way to do the same with R. I thought creating a vector containing the names and then using a for
cycle by moving the i
with [1], [2]
etc., but it doesn't work, it stops at the read.delim line not finding the file in the wd.
Here's the manipulation code I wrote. After the comment, it will repeat itself 6 times with the 6 databases I'm using (GG97 GG100 SILVA97 SILVA100 NCBI RDP).
PLUS, I repeat this process 4 times because I have 4 metrics to use (here I'm showing shannon, but I also have a copy of the code for chao1, observed_species and PD_whole_tree).
library(tidyverse)
library(labelled)
mapfile <- read.delim(file="mapfile_HC+BV.txt", check.names=FALSE);
mapfile <- mapfile[,c(1,4)]
colnames(mapfile) <- c("SampleID","Pathology_group")
#GG97
collated <- read.delim(file="alpha_diversity/GG97_shannon.txt", check.names=FALSE);
collated <- tail(collated,5); collated <- collated[,-c(1:3)]
collated_reorder <- collated[,match(mapfile[,1], colnames(collated))]
labels <- t(mapfile)
colnames(collated_reorder) <- labels[2,]
mean <- colMeans(collated_reorder, na.rm = FALSE, dims = 1)
mean = as.matrix(mean); mean <- t(mean)
GG97_shannon <- as.data.frame(rbind(labels[2,],mean))
GG97_shannon <- t(GG97_shannon);
DB_type <- list(DB = "GG97"); DB_type <- rep(DB_type, 41)
GG97_shannon <- as.data.frame(cbind(DB_type,GG97_shannon))
colnames(GG97_shannon) <- c("DB","Group","value")
rm(collated,collated_reorder,DB_type,labels,mean)
Here I paste all the outputs together, freeze the order and make the boxplot.
alpha_shannon <- as.data.frame(rbind(GG97_shannon,GG100_shannon,SILVA97_shannon,SILVA100_shannon,NCBI_shannon,RDP_shannon))
rownames(alpha_shannon) <- NULL
rm(GG97_shannon,GG100_shannon,SILVA97_shannon,SILVA100_shannon,NCBI_shannon,RDP_shannon)
alpha_shannon$Group = factor(alpha_shannon$Group, unique(alpha_shannon$Group))
alpha_shannon$DB = factor(alpha_shannon$DB, unique(alpha_shannon$DB))
library(ggplot2)
ggplot(data = alpha_shannon) +
aes(x = DB, y = value, colour = Group) +
geom_boxplot()+
labs(title = 'Shannon',
x = 'Database',
y = 'Diversity') +
theme(legend.position = 'bottom')+
theme_grey(base_size = 16)
How do I keep this code "DRY" and don't need 146 rows of code to repeat the same things over and over? Thank you!!
You didn't provide a Minimal reproducible example, so this answer cannot guarantee correctness.
An important point to note is that you use rm(...)
, so this means some variables are only relevant within a certain scope. Therefore, encapsulate this scope into a function. This makes your code reusable and spares you the manual variable removal:
process <- function(file, DB){
# -> Use the function parameter `file` instead of a hardcoded filename
collated <- read.delim(file=file, check.names=FALSE);
collated <- tail(collated,5); collated <- collated[,-c(1:3)]
collated_reorder <- collated[,match(mapfile[,1], colnames(collated))]
labels <- t(mapfile)
colnames(collated_reorder) <- labels[2,]
mean <- colMeans(collated_reorder, na.rm = FALSE, dims = 1)
mean = as.matrix(mean); mean <- t(mean)
# -> rename this variable to a more general name, e.g. `result`
result <- as.data.frame(rbind(labels[2,],mean))
result <- t(result);
# -> Use the function parameter `DB` instead of a hardcoded string
DB_type <- list(DB = DB); DB_type <- rep(DB_type, 41)
result <- as.data.frame(cbind(DB_type,result))
colnames(result) <- c("DB","Group","value")
# -> After the end of this function, the variables defined in this function
# vanish automatically, you just need to specify the result
return(result)
}
Now you can reuse that block:
GG97_shannon <- process(file = "alpha_diversity/GG97_shannon.txt", DB = "GG97")
GG100_shannon <- process(file =...., DB = ....)
SILVA97_shannon <- ...
SILVA100_shannon <- ...
NCBI_shannon <- ...
RDP_shannon <- ...
Alternatively, you can use looping structures:
General-purpose for
:
datasets <- c("GG97_shannon", "GG100_shannon", "SILVA97_shannon",
"SILVA100_shannon", "NCBI_shannon", "RDP_shannon")
files <- c("alpha_diversity/GG97_shannon.txt", .....)
DBs <- c("GG97", ....)
result <- list()
for(i in seq_along(datasets)){
result[[datasets[i]]] <- process(files[i], DBs[i])
}
mapply
, a "specialized for
" for looping over several vectors in parallel:
# the first argument is the function from above, the other ones are given as arguments
# to our process(.) function
results <- mapply(process, files, DBs)