I have a stack of images and I am trying to normalise them to unity, so that for each image the maximum is 1. How would you do it?
I'm running ImageJ on OSX, and I don't have the plugin Stacks > Normalize Stack. Thanks!
You could use the "Enhance Contrast" function in the "Process" menu and tick the "Normalize" box. Note that it doesn't work for multi-channel hyperstacks, so you have to split the channels before, and you need to convert your stack into 32 bit mode (Image > Type > 32-bit).
To properly normalize the stack, set the percentage for saturated pixels to "0" and make sure to tick the "Process all ## slices" box as well.
See the ImageJ User Guide's section about "Enhance Contrast" for more details.