library(affy)
microarrays <- ReadAffy() # 98 CEL files are read into the same object
RNAdeg <- AffyRNAdeg(microarrays)
Now I want to plot subsets of RNAdeg
plotAffyRNAdeg(RNAdeg[.......?]) # What can I do?
I've tried various 'for' loops without success.
But if plot line colors are specified then plotAffyRNAdeg plots a subset of 1:(number of colors specified), but I haven't thought of a way to use that effectively. For example, below plots the first through the sixth AffyRNAdeg'd set of microarray data (first through sixth .CEL file read in by ReadAffy() )
plotAffyRNAdeg(RNAdeg,col=c(2,2,2,3,3,3))
OK, one way was found by running AffyRNAdeg() on subsets of the object the CEL files are in and putting the resulting data in a list of lists organized by experiment, then plotting the list elements. Maybe there is an easier way, but this worked (I'm quite new to R).
library(affy)
library(RColorBrewer)
> sampleNames(ARTHwoundMA[,11:14])
[1] "GSE18960_05_GSM469416_trt_rep2.CEL" "GSE18960_06_GSM469418_trt_rep3.CEL"
[3] "GSE5525_GSM128715_ctrl12h.CEL" "GSE5525_GSM128716_ctrl24h.CEL
# RNA DEG
# Indices to subset by experiment
cel_names <- substr(sampleNames(ARTHwoundMA),1,7)
unique_exp <- unique(substr(sampleNames(ARTHwoundMA),1,7))
exp_ind <- list()
for (i in 1:length(unique_exp))
{
tempvec <- vector()
for (j in 1:length(cel_names))
{
if (cel_names[j]==unique_exp[i])
{
tempvec <- append(tempvec,j)
}
}
exp_ind[[(length(exp_ind)+1)]] <- tempvec
}
# Calculating
RNAdeg_exp <- list()
for(i in 1:length(exp_ind))
{
RNAdeg_exp[[i]] <- AffyRNAdeg(ARTHwoundMA[,exp_ind[[i]]])
}
# Plotting
colors <- colorRampPalette(rev(brewer.pal(9, "Reds")))(length(exp_ind[[i]])
pdf(file="C:\\R working directory\\TEST\\RNAdeg_plots.pdf")
for(i in 1:length(exp_ind))
{
par(bg="gray")
colors <- colorRampPalette(rev(brewer.pal(9, "Reds")))(length(exp_ind[[i]]))
plotAffyRNAdeg(RNAdeg_exp[[i]], col=colors)
plot.new()
legend("topleft", lty=1, lwd=2,col=colors,
legend=paste(sampleNames(ARTHwoundMA[,exp_ind[[i]]])))
}
dev.off()