I want to backup all the users permission and web parts on my sharepoint, so I can restore them anytime. because few weeks ago, all of user permission and web parts on my sharepoint has gone mysteriously. I've backup all the content, but not the user permissions and web parts. Can it done without using 3rd party software?
Why don't you use regular site collection backups? It includes permissions and web parts: http://technet.microsoft.com/en-us/library/ff607901.aspx
Alternatively you can make regular content database backups: http://msdn.microsoft.com/en-us/library/ms191304(v=sql.105).aspx It will also contains all permissions and web parts.